Movie EV2 Legend:
Live-cell imaging of autophagosomes in shLRBA HeLa cells. Cells were transfected with GFP-LC3 plasmid and treated with 100 nM Bafilomycin A1 for 2 hours prior to microscopy. Quantitative analyses of autophagosome size and velocity are shown in Figure 5E and 5F. Compared to shControl cells (Expanded View Movie #1), enlarged autophagosomes were observed more frequently.